Bill Gates has topped The Forbes 400 list again this year, with a net worth of $81 billion. This is the 21st year in a  row that Bill Gates is voted as the richest American.

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The bar was set high this year with a price entry of $1.55 billion, which resulted in 113 U.S. billionaires not making the list.

Second place, with a net worth of $67 billion, went to Warren Buffett who is the chief executive of Berkshire Hathaway.

Ex-CEO and founder of Oracle, Larry Ellison came third, with a net worth of $50 billion.

Elizabeth Holmes, the youngest self-made female billionaire, is also the youngest woman on the list, with a net worth of $4.5 billion. She is only 30 years old and she has already built a blood testing company, Theranos, which is valued at $9 billion.

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Facebook’s Mark Zuckerberg has moved up to 11th on the list and is also the biggest gainer on the list. However, founder of GoPro, Nick Woodman has jumped the highest with a 200% increase in his net worth since last year.

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George Lucas, Donald Trump, Steven Spielberg and Oprah made it onto the list as well.

27 new American billionaires made it onto the list, 26 people fell off and 6 people passed away. All 400 of the richest Americans together are worth a massive $2.29 trillion!